Divizia B 2000-2001 a fost a 61-a ediție a Diviziei B, al doilea eșalon fotbalistic al României.

Echipele de pe primul loc din fiecare serie au promovat direct în Divizia A la finalul sezonului, cele de pe locul secund au jucat un baraj de promovare, în timp ce șase echipe din fiecare serie au retrogradat în Divizia C.

Seria I

{| class="toccolours" border="1" cellpadding="4" style="border-collapse: collapse; margin:0;" |- bgcolor=white !width=15|<div style="text-align:center"> Loc !width=120|<div style="text-align:center"> Club !width=30|<div style="text-align:center"> M !width=30|<div style="text-align:center"> V !width=30|<div style="text-align:center"> E !width=30|<div style="text-align:center"> Î !width=30|<div style="text-align:center"> GM !width=30|<div style="text-align:center"> GP !width=30|<div style="text-align:center"> DG !width=30|<div style="text-align:center"> Pct. !width=150|Promovare sau retrogradare |- bgcolor=#D0F0C0 | 1 |Sportul Studențesc București |<div style="text-align:center"> 34 |<div style="text-align:center"> 25 |<div style="text-align:center"> 6 |<div style="text-align:center"> 3 |<div style="text-align:center"> 71 |<div style="text-align:center"> 17 |<div style="text-align:center"> +54 |<div style="text-align:center"> 81 ||<div style="text-align:center"> Promovare în Divizia A |- bgcolor=#D1E231 | 2 |Farul Constanța |<div style="text-align:center"> 34 |<div style="text-align:center"> 23 |<div style="text-align:center"> 5 |<div style="text-align:center"> 6 |<div style="text-align:center"> 58 |<div style="text-align:center"> 29 |<div style="text-align:center"> +29 |<div style="text-align:center"> 74 ||<div style="text-align:center"> Baraj pentru următorul sezon de Divizia A

| 3 |CS Năvodari |<div style="text-align:center"> 34 |<div style="text-align:center"> 19 |<div style="text-align:center"> 5 |<div style="text-align:center"> 10 |<div style="text-align:center"> 54 |<div style="text-align:center"> 31 |<div style="text-align:center"> +23 |<div style="text-align:center"> 62

| 4 |Laminorul Roman |<div style="text-align:center"> 34 |<div style="text-align:center"> 18 |<div style="text-align:center"> 6 |<div style="text-align:center"> 10 |<div style="text-align:center"> 56 |<div style="text-align:center"> 40 |<div style="text-align:center"> +16 |<div style="text-align:center"> 60 |- | 5 |Fulgerul Bragadiru |<div style="text-align:center"> 34 |<div style="text-align:center"> 14 |<div style="text-align:center"> 13 |<div style="text-align:center"> 7 |<div style="text-align:center"> 43 |<div style="text-align:center"> 35 |<div style="text-align:center"> +8 |<div style="text-align:center"> 55 |- | 6 |Metalul Plopeni |<div style="text-align:center"> 34 |<div style="text-align:center"> 15 |<div style="text-align:center"> 8 |<div style="text-align:center"> 11 |<div style="text-align:center"> 39 |<div style="text-align:center"> 34 |<div style="text-align:center"> +5 |<div style="text-align:center"> 53

| 7 |Poiana Câmpina |<div style="text-align:center"> 34 |<div style="text-align:center"> 15 |<div style="text-align:center"> 6 |<div style="text-align:center"> 13 |<div style="text-align:center"> 39 |<div style="text-align:center"> 36 |<div style="text-align:center"> +3 |<div style="text-align:center"> 51 |- | 8 |CS Metrom Brașov |<div style="text-align:center"> 34 |<div style="text-align:center"> 14 |<div style="text-align:center"> 9 |<div style="text-align:center"> 11 |<div style="text-align:center"> 33 |<div style="text-align:center"> 30 |<div style="text-align:center"> +3 |<div style="text-align:center"> 51 |- | 9 |Tractorul Brașov |<div style="text-align:center"> 34 |<div style="text-align:center"> 14 |<div style="text-align:center"> 8 |<div style="text-align:center"> 12 |<div style="text-align:center"> 45 |<div style="text-align:center"> 35 |<div style="text-align:center"> +10 |<div style="text-align:center"> 50 |- | 10 |FC Onești |<div style="text-align:center"> 34 |<div style="text-align:center"> 14 |<div style="text-align:center"> 5 |<div style="text-align:center"> 15 |<div style="text-align:center"> 43 |<div style="text-align:center"> 49 |<div style="text-align:center"> -6 |<div style="text-align:center"> 47

| 11 |ARO Câmpulung |<div style="text-align:center"> 34 |<div style="text-align:center"> 14 | 4 |<div style="text-align:center"> 16 |<div style="text-align:center"> 41 |<div style="text-align:center"> 44 |<div style="text-align:center"> -3 |<div style="text-align:center"> 46

|12 |Unirea Focșani |<div style="text-align:center"> 34 |<div style="text-align:center"> 11 |<div style="text-align:center"> 12 |<div style="text-align:center"> 11 |<div style="text-align:center"> 34 |<div style="text-align:center"> 24 |<div style="text-align:center"> +10 |<div style="text-align:center"> 45

|13 |Apemin Borsec |<div style="text-align:center"> 34 |<div style="text-align:center"> 13 |<div style="text-align:center"> 6 |<div style="text-align:center"> 15 |<div style="text-align:center"> 40 |<div style="text-align:center"> 45 |<div style="text-align:center"> -5 |<div style="text-align:center"> 45

| 14 |Cimentul Fieni |<div style="text-align:center"> 34 |<div style="text-align:center"> 12 |<div style="text-align:center"> 7 |<div style="text-align:center"> 15 |<div style="text-align:center"> 44 |<div style="text-align:center"> 44 |<div style="text-align:center"> 0 |<div style="text-align:center"> 43 |- bgcolor=#FFCCCC |15 |Callatis Mangalia |<div style="text-align:center"> 34 |<div style="text-align:center"> 9 |<div style="text-align:center"> 8 |<div style="text-align:center"> 17 |<div style="text-align:center"> 38 |<div style="text-align:center"> 50 |<div style="text-align:center"> -12 |<div style="text-align:center"> 35 |rowspan="4"|<div style="text-align:center">Retrogradare în Divizia C |- bgcolor=#FFCCCC | 16 |Juventus București |<div style="text-align:center"> 34 |<div style="text-align:center"> 8 |<div style="text-align:center"> 6 |<div style="text-align:center"> 20 |<div style="text-align:center"> 42 |<div style="text-align:center"> 63 |<div style="text-align:center"> -21 |<div style="text-align:center"> 30 |- bgcolor=#FFCCCC | 17 |Precizia Săcele |<div style="text-align:center"> 34 |<div style="text-align:center"> 5 |<div style="text-align:center"> 5 |<div style="text-align:center"> 24 |<div style="text-align:center"> 24 |<div style="text-align:center"> 62 |<div style="text-align:center"> -38 |<div style="text-align:center"> 20 |- bgcolor=#FFCCCC | 18 |Politehnica Iași |<div style="text-align:center"> 34 |<div style="text-align:center"> 2 |<div style="text-align:center"> 3 |<div style="text-align:center"> 29 |<div style="text-align:center"> 20 |<div style="text-align:center"> 96 |<div style="text-align:center"> -76 |<div style="text-align:center"> 9

Seria II